Commentary
Deciphering the molecular mechanisms underlying the pathogenesis of NASH requires loss- or gain-of-function experimental approaches. Adenovirus is a fast, effective, and versatile tool that allows transient knockdown, knockout, or overexpression of genes of interest in primary hepatocytes in vitro and in mouse liver in vivo.
Y. Liu et al. (Huazhong University of Science and Technology, China, and Harvard Medical School, USA) describe a protocol to generate adenovirus for basic medical research, and discuss critical steps during virus propagation and purification.
