Purification and isolation of hepatic stellate cells

Commentary

In an activated form, human hepatic stellate cells are the drivers of fibrosis following chronic liver injury. In NASH specifically, activated human hepatic stellate cells are drivers of induction and progression of fibrogenesis. Isolation and purification of hepatic stellate cells from donor liver samples provides an avenue to study human hepatic stellate cells and their fibrotic capabilities.

In this article, S. Lele et al. (School of Medicine, Virginia Commonwealth University, USA) described protocols for isolation of human and rodent human hepatic stellate cells.