Culture of mouse liver ductal organoids

Commentary

3D organoid culture has become a powerful tool and model for various human diseases, including NASH. The hepatic progenitor cells can be induced to form hepatic organoids, passaged or cryopreserved for future use, and can be manipulated to study the disease progression of NASH-related fibrosis.

Here, J. Yan et al. (Medical College of Virginia, Virginia Commonwealth University, Central Virginia Veterans Health System, USA) describe a protocol to establish mouse liver ductal organoids.