Fatty acid beta oxidation is a predominant bioenergetic pathway in mammals. Substantial investigations have demonstrated that fatty acid beta oxidation activity is dysregulated in many pathophysiological conditions including NASH.
In this article, W. Wang et al. (Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, Virginia, USA) describe details of practical protocols for measurement of basal and genetically or pharmacologically regulated fatty acid beta oxidation activities in the mammalian system.
The authors also discuss the advantages and disadvantages of these assays in the context of experimental purposes